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KMID : 0881720000150030241
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Journal of Food Hygiene and Safety
2000 Volume.15 No. 3 p.241 ~ p.247
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Characterization of Verotoxin-producing Escherichia coli Isolated from Domestic Foods
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곽효선:Kwak Hyo-Sun
차진:/강길진:Kang kil-Jin/김훈:Kim Hun/박선희:Park Sun-Hee/김창민:Kim Chang-MIn
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Abstract
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The incidence of verotoxin-producing Escherichia coli(VTEC) was surveyed in domestic foods including hamburger, raw meats and vegetables from 1997 to 1999. The molecular biological characteristics of the isolates were analyzed. Three VTEC strain were isolated from 1,700 samples. Serotypes of those isolates were 0157 : H7, 026 H4, and 056 : Hl2, respectively. Serotype O26 : H4 produced VT I and VT II, and 055 Hl2 isolate produced VT I, however the 60 MDa plasmid DNA and eae gene were not found from both strains. One 0157 : H7 isolate produced VT II and harbour 60 MDa plasmid DNA, however eae gene was not found in the strain. Although they produced VT, it seemed that the virulence of two strains were relatively weak because of the lack of the eae gene. In addition, the serotype O157 : H7 isolate resistant to ampicillin and streptomycin, while isolates of serotype O26 : H4 and O55 : Hl2 were multi-resistant to antibiotics including ampicillin, carbenicillin , cephalothin, trimethoprim/sulfamethoxazole and tetracycline. Supernatants of cultures of all three isolates were showed cytotoxic effect to vero and HeLa cell
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KEYWORD
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Verotoxin-producing E. coli, Serotyping, Virulence factor, Antibiotic resistance
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